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KMID : 0613820070170070970
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Journal of Life Science
2007 Volume.17 No. 7 p.970 ~ p.975
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Isolation and Characterization of Lactobacillus brevis AML15 Producing γ-Aminobutyric Acid
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Shin Ji-Won
Kim Dong-Geol
Lee Yong-Woo
Lee Hyung-Seok
Shin Kee-Sun
Choi Chung-Sig
Kwon Gi-Seok
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Abstract
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For the screening of γ-aminobutyric acid (GABA)-producing bacteria, 86 bacterial strains which produce GABA were isolated from Kimchi and Salted fish. Among these, three strains designated AML15, AML45-1, AML72 with relatively high GABA productivity were selected by thin layer chromatography (TLC). To elucidate the relationship between isolated strains and the genus Lactobacillus, their 16S rDNA sequence were examined. The result of their DNA sequences showed 99% similarity with Lactobacillus brevis ATCC 367. On the basis of the these results, isolated strains were identified as Lactobacillus brevis and designated L. brevis AML15. In order to determine the optimum conditions for GABA production, the isolated strains were cultivated in pyridoxal phosphate (PLP) and monosodium glutamic acid (MSG). Results showed that L. brevis AML15 had the highest GABA productivity with 10,424 nM/μl concentration in MRS broth containing 5% (w/v) MSG and 10 μM PLP at pH 5.0. The results imply that L. brevis AML15 has the potential to be developed as a strain for GABA hyper-production.
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KEYWORD
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γ-Aminobutyric acid, Glutamate decarboxylase, Lactobacillus brevis
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